Fluorescent Proteins II: Application of Fluorescent Protein Technology (Springer Series on Fluorescence)

Fluorescent proteins are in detail hooked up to investigate within the existence sciences. Tagging of gene items with fluorescent proteins has revolutionized all parts of biosciences, starting from primary biochemistry to scientific oncology, to environmental learn. the invention of the golf green Fluorescent Protein, its first, seminal software and the creative improvement of a vast palette of fluorescence proteins of alternative shades, was once accordingly regarded with the Nobel Prize for Chemistry in 2008.  

Fluorescent Proteins II highlights the physicochemical and biophysical elements of fluorescent protein know-how past imaging. it really is adapted to fulfill the wishes of physicists, chemists and biologists who're drawn to the basic homes of fluorescent proteins, whereas additionally focussing on particular functions. The implementations defined are state-of-the-art experiences and exemplify how the actual and chemical homes of fluorescent proteins can stimulate novel findings in lifestyles sciences.

Show description

Quick preview of Fluorescent Proteins II: Application of Fluorescent Protein Technology (Springer Series on Fluorescence) PDF

Similar Biology books

10% Human: How Your Body's Microbes Hold the Key to Health and Happiness

You're simply 10% human. for each one of many cells that make up the vessel that you just name your physique, there are 9 impostor cells hitching a journey. you're not simply flesh and blood, muscle and bone, mind and epidermis, but additionally micro organism and fungi. Over your lifetime, you'll hold the identical weight of 5 African elephants in microbes.

Who's in Charge?: Free Will and the Science of the Brain

“Big questions are Gazzaniga’s inventory in exchange. ”—New York Times“Gazzaniga is without doubt one of the so much fabulous experimental neuroscientists on the earth. ”—Tom Wolfe“Gazzaniga stands as a huge between neuroscientists, for either the standard of his learn and his skill to speak it to a basic public with infectious enthusiasm.

Fragment Based Drug Design, Volume 493: Tools, Practical Approaches, and Examples (Methods in Enzymology)

There are various first-class studies on fragment-based drug discovery (FBDD), yet there are to this point no hand-holding courses or protocols with which you could embark in this orthogonal method of supplement conventional excessive throughput screening methodologies. This equipment in Enzymology quantity deals the instruments, sensible methods, and hit-to-lead examples on easy methods to behavior FBDD monitors.

The Invisible History of the Human Race: How DNA and History Shape Our Identities and Our Futures

• A ny Times Notable e-book •“The richest, most up to date, most enjoyable e-book on genetics in your time. ” —The manhattan occasions booklet overview we're doomed to copy background if we fail to profit from it, yet how are we stricken by the forces which are invisible to us? within the Invisible historical past of the Human Race Christine Kenneally attracts on state of the art study to bare how either old artifacts and DNA let us know the place we come from and the place we can be going.

Additional info for Fluorescent Proteins II: Application of Fluorescent Protein Technology (Springer Series on Fluorescence)

Show sample text content

Biol J Okayama collage 2:11–21 88. Logan A, Halcrow ok, Tomascik T (1990) UV excitation-fluorescence in polyp tissue of convinced scleractinian corals from Barbados and Bermuda. Bull Mar Sci 46:807–813 89. Wiedenmann J, Lorenz U (2000) Fluoreszenz- und elektronenemikroskopische untersuchungen an der seeanemone Anemonia sulcata Pennant (Cnidaria, Anthozoa). Mikrokosmos 89:1–6 ninety. Vogt A, D’Angelo C, Oswald F, Denzel A, Mazel CH, Matz M, Ivanchenko S, Nienhaus GU, Wiedenmann J (2008) A eco-friendly fluorescent protein with photoswitchable emission from the deep sea. PLoS ONE 3:e3766 ninety one. Nienhaus ok, Renzi F, Vallone B, Wiedenmann J, Nienhaus GU (2006) Chromophore-protein interactions within the anthozoan eco-friendly fluorescent protein asFP499. Biophys J 91:4210–4220 ninety two. Nienhaus GU (2010) The “wiggling and jiggling of atoms” resulting in excited-state proton move in eco-friendly fluorescent protein. Chemphyschem 11:971–974 ninety three. Ando R, Mizuno H, Miyawaki A (2004) Regulated quickly nucleocytoplasmic shuttling saw through reversible protein highlighting. technology 306:1370–1373 ninety four. Verkhusha VV, Chudakov DM, Gurskaya NG, Lukyanov S, Lukyanov KA (2004) universal pathway for the pink chromophore formation in fluorescent proteins and chromoproteins. Chem Biol 11:845–854 ninety five. Baird GS, Zacharias DA, Tsien RY (2000) Biochemistry, mutagenesis, and oligomerization of DsRed, a purple fluorescent protein from coral. Proc Natl Acad Sci united states 97:11984–11989 ninety six. Cotlet M, Hofkens J, Habuchi S, Dirix G, Van Guyse M, Michiels J, Vanderleyden J, De Schryver FC (2001) identity of alternative emitting species within the purple fluorescent protein DsRed via ensemble and single-molecule spectroscopy. Proc Natl Acad Sci united states 98:14398–14403 ninety seven. Prescott M, Ling M, Beddoe T, Oakley AJ, Dove S, Hoegh-Guldberg O, Devenish RJ, Rossjohn J (2003) the two. 2 A crystal constitution of a pocilloporin pigment unearths a nonplanar chromophore conformation. constitution 11:275–284 ninety eight. Andresen M, Wahl MC, Stiel AC, Grater F, Schafer LV, Trowitzsch S, Weber G, Eggeling C, Grubmuller H, Hell SW, Jakobs S (2005) constitution and mechanism of the reversible photoswitch of a fluorescent protein. Proc Natl Acad Sci united states 102:13070–13074 ninety nine. Wang Z, Shah JV, Chen Z, solar CH, Berns MW (2004) Fluorescence correlation spectroscopy research of a GFP mutant-enhanced cyan fluorescent protein and its tubulin fusion in residing cells with two-photon excitation. J Biomed choose 9:395–403 a hundred. Chattoraj M, King BA, Bublitz GU, Boxer SG (1996) Ultra-fast excited country dynamics in eco-friendly fluorescent protein: a number of states and proton move. Proc Natl Acad Sci united states 93:8362–8367 a hundred and one. Brejc okay, Sixma TK, Kitts PA, Kain SR, Tsien RY, Ormo M, Remington SJ (1997) Structural foundation for twin excitation and photoisomerization of the Aequorea victoria eco-friendly fluorescent protein. Proc Natl Acad Sci united states 94:2306–2311 102. Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED (2003) Mechanism and energetics of eco-friendly fluorescent protein chromophore synthesis printed by way of trapped intermediate constructions.

Download PDF sample

Rated 4.93 of 5 – based on 43 votes